544 thousand. The investment in opening a seafood cafeteria includes the initial fee of 68,000 yuan, the brand use fee of 0 yuan, the deposit of 1 1,000 yuan, the equipment cost of 36,000 yuan, the first batch of materials cost of about 4 1 1,000 yuan, the decoration cost of 70,000 yuan, the marketing cost of 8,000 yuan, and the basic cost of 320,000 yuan. These expenses add up to 544,000 yuan.