In vitro anther culture is a tissue culture technique, and its process is as follows: (1) The anthers whose pollen has developed to a certain stage are inoculated on artificial culture medium for in vitro culture; (2) Pollen can divide many times under the specific conditions provided by the culture medium to form embryoid structure (embryoid) or callus; ③ Induce callus to differentiate into buds and roots, and finally grow into plants. Haploid breeding is a kind of breeding method, and its process is: on the basis of anther culture in vitro, haploid seedlings are treated with colchicine to double the chromosome number and restore it to twice. Because their diploid chromosomes are doubled from haploid chromosomes, they are all pure lines, and their self-bred offspring will not separate, so they have high application value in breeding.