English name Flow Cytometer
Domestic/import import
Origin/brand Beckman Coulter Co., Ltd.
A classic epic? ALTRA？
Information quotation
Seller Beckman Coulter Hong Kong Limited
flow cytometry
performance parameter
Epic? ALTRA？ Flow cytometer with super-sorting cell sorting
Data management
The software systems FlowCentreTM and EXPOTM32 built on the Windows platform have established a powerful multi-task working environment, which can be used for data collection, analysis and result reporting.
Selection of sorter
Small and economical air-cooled laser can speed up sorting to 10000/s, which provides sufficient space for most application fields. If your purchasing power permits, or research work needs it, the HyPerSort system will provide more efficient and accurate sorting results at a higher sorting speed.
Simple design
The operator can directly check or replace the filter to obtain a wider combination of fluorescent dyes without recalibrating the optical path.
Configuration optics
ALTRA multi-light source selection provides the most matching space for users' current and future research work.
Multiple lasers
Optical table and industry standard components allow multiple lasers to be combined to support application work. Economical air-cooled laser and high-power water-cooled laser provide users with many choices, and users can easily use them or switch between multiple beams.
Shi Ying flow cell
Beckman Coulter is the first company in the industry to design a high-sensitivity timely flow cell SortSense, which allows users to use any parameter, including side scattered light, in standard and high-speed sorting modes.
Intelligent Soting
The brand-new ALTRA sorting mode ensures the purity of 99% on the premise of ensuring the output. Using the AccuSORT mode, a specific number of cells can be separated into different wells of the microplate. It is very convenient to switch between standard sorting mode and high-speed sorting mode.
Sorting lock
SortLOCK function truly realizes sorting without waiting, and the monitoring screen intuitively displays the sorting monitoring status in real time, thus ensuring the credibility of sorting results.
specifications
Detection parameter
Forward Logarithm, Integer and Peak
PMT 1—LOG，INT
PMT2-PMT 5-LOG，INT，PEAK
Two additional signal channels (AUX) can be connected to pmt2-pmt5; Casual; Optional gate amplifier function
Parameters can be assigned to any peak signal.
1043 inch digital amplifier
data acquisition
Among these 27 parameters, 12 parameters can be collected synchronously.
Forward scattering signal
Two auxiliary signals
Time time parameter
proportion
prism
Laser selection
Air cooled laser
488 nm argon ion laser
MellesGriot He-Ne laser, 633 nm, 10mW.
All-chromium He-Ne laser, 325 nm, 20mW
MellesGriot He-Ne laser, 544 nm, 1.5 MW
Coherent enterprise II627 argon ion laser, ultraviolet (50mW), 488nm( 180mW).
Optional air-cooled operating system with LASERPURE 5i.
Coherentlnnova 70 series, 90 series and 300 series lasers.
Flow chamber
76 ? Timely flow chamber
100, 140 timely flow cell
250 ? M biological hazard timely flow chamber
5 1, 6 1, 76, 100 and 150? Air jet
Flow cham of high-speed sorting system
70? M and 76? Timely flow chamber
Beam shaper and photomultiplier tube
Spot size (? M) height x width (? m)
12x5 1 15x60
12x 15 1 15x 80
12x70* 15x80
8x 15 1* 10x80
6.5x 15 1 8x80
6 x 1 12 15x80
17x34 40x80
* Optional.
The spectral detection range of photomultiplier tube (PMT) is 300-800nm, and the voltage adjustment range is 0-2000 V.
sample cell
Sample tubes of various specifications are selected: 12X75, 12X76, 13X 100, which ensures mass production of samples.
In the process of sample classification, avoid frequent replacement of sample tubes.
size
Provide standard kit
The separation purity is greater than 99%, and the speed premise of this separation purity is 10000 SortSense.
Compared with 9 separation modes (including 3 ALTRASort separation modes) of 15000 air jet, it simultaneously provides more than 99% purity, more than 99% recovery rate and more than 98% viability. Enrichment mode and AccuSortTM accurate counting mode are used to establish sorting matrix and automatic cloning.
HyPerSort system can be used as an option.
When the speed is 25000/s, the separation purity is over 98%, the recovery rate is over 95% and the survival rate is over 97%.
sensitive
& lt 100MESF(Spherotech beads)
The price is 640 thousand