Saiye Biotechnology has been focusing on the systematic research of stem cells for many years, and established a series of research methods and quality standards including cell extraction, separation, purification, identification, in vitro differentiation, in vivo differentiation, tracing, cell transgene, function and mechanism research, which has become one of the internationally recognized standards in the field of stem cells.
Saiye Bio has established a powerful molecular biology technology platform and formed an all-round technical service system by using its own technological advantages. Around gene expression, gene silencing and RNA interference (RNAi), microRNA and other technologies, it provides dozens of technical services including gene cloning, plasmid/vector construction, lentivirus (LV), adenovirus (AV), retrovirus (MLV), cell transfection, PCR, RT-PCR, QCPR, Westernblot, Elisa and so on.
Cyagen Transgenic Animal Center is the technical service platform of transgenic model animal with the strongest technical service capability and the highest service efficiency in China, and it is also one of the most mature commercial transgenic animal technical service platforms in the world. At present, the platform builds more than 500 transgenic mouse strains for domestic and foreign customers every year, and customers are all over the world.
At present, Saiye Bio is building an SPF model animal Center, which is five times as large as it is now. The project will be put into production at the end of 20 1 1. By then, more than 3,000 transgenic mice/rats and knockout mice/rats will be built every year.
The technical team of bioinformatics developed the first online carrier design software "Vectorbuilder" (website: direct online search) with international leading technology and unique innovative ideas, and summarized the complex carriers into six systems, on which almost any demand can be quickly found! The software version 1.0 has attracted wide attention in the industry since it came out in the first half of 20 10. After more than a year of promotion and improvement, the powerful version 2.0 software was launched at the end of September, 20 1 1! Next, Saiye Bioinformatics team will influence the whole life science field from a broader perspective and a deeper level.
Drug screening platform based on GPCR specific map detection technology
G protein-coupled receptor (GPCR) is the largest family of membrane proteins in human cells, with about 800 members. These membrane protein receptors specifically recognize and bind small molecular ligands outside the cell (such as photons, ions, lipids, polypeptides, nucleosides, nucleotides, neurotransmitters and peptide hormones), and transmit signals to the inside of the cell through G proteins coupled with them in the cell membrane. GPCR is involved in a wide range of biological processes and plays a vital role in human life activities, such as memory behavior, people's response to visual, olfactory and taste information and blood pressure regulation. And participated in the occurrence and development of many major diseases, such as HIV invasion, cancer metastasis, asthma, hypertension, diabetes, arthritis, allergic inflammation, depression and so on.
Up to now, more than 50% of drugs approved by FDA in the United States are aimed at GPCR. GPCR spectrum analysis analyzes the specific recognition and action properties of lead compounds with various gpcrs, infers their potential toxic and side effects, and determines whether they will enter expensive preclinical and clinical trials. At present, this method has become the most widely used detection index in new drug research. In addition, because the GPCR data show the specificity and even safety of the compounds, FDA and SFDA need this set of data when applying for new drugs, regardless of whether the candidate drugs are aimed at GPCR or any other types of targets, such as enzyme targets and channel targets.
At the beginning of 20 10, Saiye Bio, Beaver Biotechnology and Sheng Yuan Pharmaceutical jointly developed a highly sensitive and stable GPCR spectrum detection platform. In this platform, complex and diverse signal pathway effectors were fused into Ca2+ by efficient transient transfection technology combined with specially constructed Gq mutant, and the changes of calcium flow were detected by * * * transfected chemiluminescence calcium ion sensor. The platform avoids the traditional GPCR stable cell line technology and has the advantages of short detection period, low cost and high efficiency. At the same time, the chemiluminescence detection scheme is adopted to avoid the noise interference of conventional fluorescence detection, and the signal-to-noise ratio is higher, which increases the accuracy and sensitivity of experimental data.
At present, BeaverBioGPCR detection platform has developed to 40 GPCR covering multiple signal pathways, which can meet the individual needs of customers, and can be used to study selectivity or potential, determine structure-activity relationship (SAR) and explore possible GPCR-mediated functions. GPCR spectral analysis allows the study of single or multiple dose responses, and can identify various compounds, including complete agonists, partial agonists, antagonists or isomer modulators. Oricel l & ampBeaver Nano3D cell culture hydrogel
Oricel L & Beaver nano-3D cell culture hydrogel is a kind of polypeptide three-dimensional cell growth scaffold synthesized from natural amino acids, which contains 0.5%-2% of polypeptide components and 98%-99.5% of water, with definite components and no toxic effect on cells. The polypeptide can be spontaneously assembled into nanofibers in aqueous solution, and can be further crosslinked under physiological conditions to form hydrogel with porous network structure, and its pore size range is 50-200nm. The product can simulate the matrix environment of cell growth to the greatest extent, promote cell growth and differentiate into specific three-dimensional tissue structure, and the experimental results obtained are closer to the state of cell growth in vivo.
Product advantages:
[1] Easy to use: the product is simple to use and operate, the material is transparent, and it is easy to observe and study the cell growth state;
[2] Accurate and reliable: realize the three-dimensional culture of cells and provide a matrix environment closer to the living conditions in vivo for cells;
[3] Safety and environmental protection: clear composition, stable performance and no toxic effect on cells; Can be naturally degraded into amino acids, and does not produce any toxic and harmful wastes.
[4] Wide application: it is suitable for the culture of different cells, and different kinds of cells can be selected according to different research purposes.
Products.
Application:
[1] Conventional cell culture experiment: It is used to improve cell activity and maintain its growth state, and has a good culture effect on some cells that are difficult to culture, such as stem cells.
[2] Neuron cell culture experiment: used to improve the activity of neuronal cells and study the function of nervous system.
[3] Experiment of hamster brain regeneration and repair: It is mainly used for adjuvant treatment of cerebral hemorrhage, cerebral thrombosis and other diseases.
[4] Three-dimensional culture experiment of chondrocytes and osteoclasts: applied to sports medicine, repair and regeneration of articular cartilage, fracture and rupture.
[5] 3D culture experiment of fibroblasts: applied to skin repair and gingival repair in medical and aesthetic industries.
S-layer protein is a membrane protein of bacteria, and its molecular weight is about 40-200kD. It has the characteristics of self-assembly, and can spontaneously form highly ordered and dense nano-protein layers on solid or other surfaces. The S-layer protein and protein A/G are fused by molecular biology technology, and the fused protein can be spontaneously coated on the surface of magnetic beads, and the A/G end of the protein can be bound to the FC fragment of the antibody outward, and the active recognition sites of the antibody are also distributed outward, thus improving the utilization rate of the antibody. It can be used to separate various objects, and has the advantages of high antibody density and high surface activity of magnetic beads. All antibodies can be combined with magnetic beads, and their own specific magnetic beads can be prepared simply. Can be used in the fields of clinical diagnosis, stem cell separation, environment and food safety; It can solve the separation problem of cell therapy and contribute to the maturity of new cell therapy.
Saiye Biotechnology is establishing an efficient, simple and self-assembled nano immunomagnetic beads and stem cell clinical application research platform with independent intellectual property rights. By using the nonspecific binding antibody of S-layer protein and A-protein fusion protein, a wide variety of immunomagnetic bead products are classified into one category, and the density and vitality of antibodies are improved by using the characteristics of self-assembly and high-density arrangement, which greatly improves the utilization rate of antibodies; It overcomes the shortcomings of traditional crosslinking technology, such as non-directionality, low density, high price and non-universality.