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How to cultivate soil microorganisms? (Soil Leachate)
Wet fresh soil can be used to cultivate bacteria and fungi.

Sun-dried soil is used to cultivate actinomycetes.

But you should use a different selection medium.

The preparation of soil leachate should be as follows: add about 30 sterilized glass beads to a certain amount of sterile water, and shake continuously for about half an hour while soaking. The supernatant obtained by standing is diluted quantitatively by pipette (generally diluted to the fifth to seventh power of 10), and then inoculated on the culture medium plate by smear method, and finally a single colony can be obtained, which is convenient for counting and identification.

Actinomycetes are cultured in the medium of Koch 1, in which starch is the carbon source.

Beef paste peptone medium is used to cultivate bacteria.

Fungi were cultured in potato glucose medium and antibiotics were added to inhibit the growth of bacteria.

Adding agarose to make a solid plate.

There are details. Welcome to send text messages to discuss at any time.

Graduate program or undergraduate interesting experiment? If it's a topic, cover your face and run. ....

But I think, at least you can screen and separate with a medium plate that only uses benzene series as carbon source, and then put it into a liquid medium for domestication, domestication and domestication, at least you can get strains instead of miscellaneous bacteria.