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The use of 1 refers to the breeding technology of animals (including mammals, insects, fish and poultry, etc.). Cell culture in vitro, that is, the method of taking out tissues or cells from the body under aseptic conditions, simulating the basic conditions of normal physiological conditions in the body, and allowing them to continue to survive, grow and reproduce in culture containers. Cells cultured in vitro can be divided into primary cells and passage cells. Animal cell culture brings great convenience to the study of cell biology, and solves the problem of taking human living cell materials as research objects or research tools; By cultivating animal cells, we can produce some products with important diagnostic and therapeutic value in medicine, such as protein and virus.
2 process: take the organs and tissues of animal embryos or young animals. Cut the material into pieces, treat (digest) with trypsin to form single cells, and put the single cells into the culture medium to make a cell suspension with a certain concentration. The dispersed cells in the suspension will quickly attach to the bottle wall and become cell adhesions. When adherent cells divide and grow into contact with each other, cells will stop dividing and proliferating, and contact inhibition will occur. At this time, the contact-inhibited cells need to be treated with trypsin again. Then prepare a cell suspension with a certain concentration. In addition, we call the culture within 10 generation as primary culture. After 10 generation, it is called subculture. Cells cultured for 10~50 generations are called cell lines. After more than 50 generations of culture, most cells have aged and died, but some cells have changed their genetic material, which has the characteristics of unlimited passage, that is, canceration. Cells at this time are called cell lines.