The basic principle of liquid suspension culture system is consistent with the above-mentioned laboratory suspension culture method, but it is used for the equipment used in large-scale culture

The preparation and control technology is much more complicated than small-scale culture in the laboratory.

Mechanical stirring culture system

Mechanical stirred bioreactor is usually improved on the basis of microbial fermentation tank according to the characteristics of plant cells.

Its equipment requires the following improvements on the basis of microbial fermentation tank: the stirring device should reduce the shear force and generally be changed to impeller type.

It is spiral; Because of the long growth cycle of plant cells, it is necessary to supplement water and nutrition at any time, so it is necessary to design a liquid adding device;

Because the physiological activities of plant cells need fresh air, and cell metabolism may also produce harmful gases, it is necessary to design ventilation.

Gas installation; ? In order to facilitate sampling observation, sampling ports are generally designed.

Air pressure stirring culture system

Considering that the shearing action of the reactor with mechanical stirring is inevitable, and the rotation of the central shaft of the stirrer is often easy to make the culture.

Therefore, the air-lift bioreactor has been developed in the polluted part of nutrients, but its disadvantage is uneven stirring.

Rotary culture system

Generally used in product trials or some cultures that need to lyse cells to obtain target products. Its advantages are accurate control,

Flexible treatment, the disadvantage is that the culture volume is small.