1. How to see the results of fluorescence quantitative PCR is calculated according to the formula: control group -Ct = control group (Ct target gene -Ct reference gene)-control group (Ct target gene -Ct reference gene).

2. How to analyze the results of fluorescence quantitative PCR (CT value) ~ The lower the CT value, the larger the initial template number ~

3. What do you think of the results of mirna fluorescence quantitative PCR? Roche's reaction speed is quite fast. After sample processing (nucleic acid extraction), it usually takes about 30 minutes to 1 hour to put it on a fluorescence quantitative PCR instrument. But if

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How to analyze electrophoresis patterns in fluorescence quantitative pcr-Baidu knows

Two answers: 2065438+April 27th, 2006.

The best answer: the brightness of internal reference is needed to compare the differences of gene expression. The practice of semi-quantitative RT-PCR reference is generally 3. After electrophoretic scanning, the gray value is calculated, and the internal reference and purpose of the same specimen are compared first. ...

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