In microbial experiments, it is often necessary to transfer the spore slant or shake flask seeds prepared in the laboratory to shake flask for expanded culture. This is because shaking bottles can provide more oxygen and nutrients, which is beneficial to the growth and reproduction of microorganisms. At the same time, the shake flask culture method is closer to the natural environment and can better simulate the growth of microorganisms.

In the process of transplanting spore slant or shake flask seeds into shake flask, the following points should be paid attention to:

Ensure aseptic operation:

The grafting process needs to ensure aseptic operation to avoid contamination by miscellaneous bacteria. You can use alcohol lamp, alcohol cotton and other tools to disinfect the surface.

Select the appropriate medium:

Shake flask culture medium should be suitable for the growth conditions of spore slant or shake flask seeds to ensure the growth and reproduction of microorganisms.

Control temperature and humidity:

Shake flask culture needs to be carried out under certain temperature and humidity conditions. The growth rate and metabolic process of microorganisms can be controlled by adjusting the rotation speed and temperature of shake flask.

Observe the growth:

In the process of shake flask culture, it is necessary to observe the growth of microorganisms regularly, including the shape, color and size of colonies, so as to find problems in time and make adjustments.

In a word, it is necessary to transfer the spore slant or shake flask seeds prepared in the laboratory to shake flask for expanded culture, so as to provide more sufficient oxygen and nutrition and promote the growth and reproduction of microorganisms. In the process of grafting, attention should be paid to aseptic operation, selection of suitable culture medium, control of temperature and humidity, and observation of growth to ensure the smooth progress of microbial experiments.

In addition, when conducting microbial experiments, we need to pay attention to the safety of the experiments. For example, it is necessary to avoid dangerous situations such as sharp instrument stabbing and infection with germs during surgery. At the same time, we should also pay attention to the treatment and discharge of experimental waste to avoid adverse effects on the environment and human beings.