B, those with nuclear membranes are: yeast, amoeba, Chlorella and Spirogyra, so B is wrong.
C, with ribosomes but no chromosomes: ① oscillatoria, ⑦ Escherichia coli, ⑨ Pneumococcus, so C is correct.
D, autotrophic is: ① Oscillatoria ④ Chlorella ⑤ Spirogyra, so D is correct.
Therefore, choose: B.