1, preparation, preparation and sterilization of culture medium (12 1- 126 degrees for 30 minutes), glassware sterilization (170 degrees for 2 hours in dry heat or wet heat sterilization) can greatly prolong the sterilization time.

2. Microbiology laboratory and aseptic operation desk turn on the ultraviolet lamp for sterilization 1 hour, and enter the aseptic room at least half an hour after closing. Usually we enter the sterile room after 1 hour. Because there is residue after ultraviolet lamp irradiation.

3. Wear special clothes, hats, shoes, masks and gloves when entering the sterile room. Contact with sterile things should be sprayed with 75% alcohol or smeared. If it is drugs, the requirements are more stringent.

4. After the experiment, put the dish upside down in an incubator with a set temperature, and observe and record it when necessary.

5. After that, the sterile room can be ventilated, otherwise the alcohol in it smells bad!

6. Just throw away the samples that are not used in the experiment! However, after observation, it is best to boil the culture medium of long bacteria, and it is best to cook the dishes.

Baidu encyclopedia-microorganism laboratory