Plant tissue culture is based on the theory that plant cells are totipotent. In vitro, organs, tissues or cells and protoplasts are used to induce callus, adventitious buds and adventitious roots, and finally complete plants are formed in sterile and suitable artificial culture medium and temperature.

Regenerated plants are obtained by cultivating various tissues of plants, such as cambium, parenchyma, mesophyll and endosperm. It also refers to the callus of various organs cultivated in the process of culture, and the callus differentiates again to form regenerated plants.

However, this tissue is not differentiated, but a group of parenchyma cells called callus. Under the conditions of suitable light, temperature and certain nutrients and hormones, callus began to differentiate.

Extended data:

According to the purpose of culture, the materials should be selected appropriately, and the selection principle is easy to induce and less bacteria. It is necessary to select sterile materials in plant tissues. On the one hand, we should take materials from strong plants, not materials with wounds or pests.

On the other hand, materials should be taken in sunny days, preferably at noon or afternoon, and never in rainy days, cloudy days or when the dew is still wet. Because in sunny days, strong plants and tissues with strong photosynthesis and respiration have disinfection effect themselves, such tissues are generally sterile.

Plant materials selected from outdoor or indoor contain various microorganisms to varying degrees. Once these pollution sources are brought into the culture medium, they will pollute the culture medium. Therefore, plant materials must undergo strict surface sterilization treatment, and then access the culture medium through aseptic operation procedures.