1, whole blood Whole blood is composed of liquid plasma and red blood cells freely suspended in plasma. It is a red, opaque and viscous liquid. Whole blood containing maintenance solution can be divided into two parts after red centrifugation or natural precipitation in a container. The upper yellow-green liquid is plasma; The lower layer is dark red opaque red blood cells with a small amount of plasma. There is a thin layer of white material between plasma and red blood cells, mainly white blood cells and platelets. Whole blood mainly has the functions of transportation, regulation, immunity, anti-unloading, coagulation, hemostasis and so on. Preservation: ACD preservation solution, whole blood can be preserved for 265,438 0 days at 4 2℃. Indications: (65,438+0) Acute massive hemorrhage: such as postpartum hemorrhage, major surgery or severe trauma, the number of red blood cells and blood solubility are obviously reduced; (2) Extracorporeal circulation: In cardiopulmonary surgery, an extracorporeal circulation machine is used, and whole blood is often used as the bottom fluid of the pump; (3) exchange blood
2. Blood composition
See attachment (1) red blood cells (2) platelets (3) white blood cells (4) plasma.
(2) Special business
1, erythrocyte blood group identification, antigen identification of ABO, Rh and other erythrocyte blood group systems. Clinical difficult blood group identification. (3) Study and analyze the relationship between erythrocyte blood group antigen and diseases. 2. Clinical significance of antibody screening and identification of red blood group system: (1) To ensure the safety of patients' blood transfusion through incomplete antibody identification; (2) Provide a targeted blood transfusion plan for clinic; (3) Laboratory diagnosis of hemolytic disease of newborn and autoimmune hemolytic disease; (4) Support the identification of red blood cell blood type; (5) Investigation of transfusion reaction. 3. Clinical significance of antibody titer analysis: (1) Understand and analyze the causes of antibody production and antibody level; (2) Support clinical selection of treatment scheme and analysis of treatment effect. 4. The significance of the difficult-to-match blood test: (1) to ensure the safety of clinical blood transfusion; (2) Discovering the existence of irregular antibodies; (3) Provide technical support for blood transfusion in primary hospitals. 5. The significance of blood group serological examination of neonatal immune hemolytic disease (HDN): (1) is helpful for clinical diagnosis; (2) Provide reasonable suggestions for clinical treatment. 6. Significance of prenatal laboratory examination of fetus: (1) Early detection of whether the fetus is at risk of HDN. (2) To provide laboratory basis for clinical evaluation of the pathogenesis of HDN, selection of treatment scheme and determination of the best delivery time. 7. The significance of laboratory examination of autoimmune hemolytic disease (AIHA): (1) is helpful for clinical diagnosis; (2) Provide help for the explanation and treatment of clinical diseases. 8. Significance of determination of soluble blood group substances: (1) is used to identify difficult blood groups or subtypes.
9. Significance of antigen-antibody examination of platelet blood group system (1) provides guarantee for the effectiveness of clinical PC infusion analysis (2) analyzes the causes of blood transfusion reaction (10), and investigates the significance of handling blood transfusion reaction: (1) analyzes the causes of blood transfusion reaction, and advises clinicians to deal with it symptomatically (2) discovers and ensures the safety of blood transfusion in the future.
The significance of 1 1, HLA-A and B site antigen examination (1) provides HLA-A and B antigen matching for clinical tissue transplantation; (2) Exclusion of parent-child relationship (3) Association analysis between HLA antigen and disease (12), HLA-B27 antigen detection (1) assisting the clinical diagnosis of ankylosing spondylitis (13), and the significance of HLA antibody screening: (1 3) Obtaining HLA typing reagents through screening. (2) Analyze the causes of patients' blood transfusion reaction; (3) transplantation and matching.