Abstract: Microhydrogel has been proved to be beneficial in many applications, such as tissue engineering and drug delivery. A key aspect of these applications is the microstructure of biological or chemical compounds in hydrogels. For this purpose, sequential pattern gel can be used for any living materials in space, tissues imitating complex organisms or various chemically designed functional particles for drug delivery. Lithography is the most common way, but the mode of micro-hydrogel photocrosslinked polymer Co., Ltd., so far, the conventional micro-method uses static mold to manufacture the structure, which can be generated by limiting the obtained shape. Here, we describe a dynamic micro-molding technology to manufacture a micro-mold based on thermally responsive poly (N- isopropylacrylamide) by using the microstructure of a continuous pattern hydrogel. These reactive micro-molds show shape changes when the temperature changes, which promotes the continuous molding of microgels at different temperatures. We produce fluorescent polymer microspheres with multi-chamber stripes, cylinders and cubes or different cell types. These reactive micro-models can be used to fix continuous pattern gel microstructure and chemical substances of biomaterials, which may be beneficial to a series of applications in interface chemistry, material science and engineering and biology.