Microscopic examination: 1 heterotrophs: heterotrophs obtained organic substances by predation, digested and absorbed these small molecules and synthesized them into its own substances, such as polysaccharides, protein, nucleic acids and lipids. This is the assimilation of heterotrophs (an ordinary animal), so its nitrogen source comes from organic matter. Autotrophic organisms refer to organisms that live and reproduce only with inorganic compounds, so their nitrogen source is inorganic substances.