Test site: energy change and chemical energy synthesis change of substances in the process of light reaction and dark reaction: analysis: 1. Autotrophic organisms: refers to most green plants and a few kinds of bacteria synthesize organic matter with light energy or chemical energy as energy source and carbon dioxide in the environment as carbon source. Metabolic types that store energy, such as green plants, photosynthetic bacteria and chemosynthetic bacteria. 2. heterotrophs: Inorganic substances cannot be directly synthesized into organic substances, and ready-made organic substances must be ingested to maintain a nutritious lifestyle. Such as animals, people, most bacteria and fungi. ① Escherichia coli can't carry out photosynthesis or chemical synthesis, and it is a heterotrophic prokaryote. ① error; ② Nitrifying bacteria can be chemically synthesized and are autotrophic prokaryotes, which is correct; ③ Yeast can't carry out photosynthesis or chemical synthesis, and it is a heterotrophic eukaryote. ③ Wrong; ④ Chlamydomonas contains chloroplasts and can carry out photosynthesis. It is an autotrophic eukaryote. 4 correct; ⑤ Chinese cabbage contains chloroplasts, which can carry out photosynthesis and is an autotrophic eukaryote. ⑤ Correct; ⑥ Mushrooms can't carry out photosynthesis or chemical synthesis, so they are heterotrophic eukaryotes. 6 wrong. So this topic examines the knowledge of metabolic types. The key to solving the problem is to understand the main differences between autotrophs and heterotrophs. The key is whether carbon dioxide can be used to synthesize carbon-containing organic compounds, so we should strengthen our understanding.