Mycoplasma is the smallest and simplest prokaryote, which can live independently. Ureaplasma urealyticum is the only confirmed ureaplasma urealyticum, which is characterized by urea-active enzyme. Mycoplasma hominis belongs to mycoplasma. These two genera belong to the same family, and their characteristics are that cholesterol or other sterols are needed for growth, and urea is needed for the growth of Ureaplasma urealyticum.
Culture characteristics: Chlamydia is an obligate intracellular parasite, which can not be cultured in artificial medium, but can be cultured in chicken embryo yolk sac, HeLa-299, BHK-2 1, McCoy and other cells. Centrifugally inoculating cell culture tubes with samples to promote chlamydia adhesion to cells; Or adding diethylaminoethyl dextran into the culture tube to enhance the adsorption of chlamydia to susceptible cells and improve the positive rate of separation and culture.
Culture characteristics of mycoplasma: it can grow in artificial culture medium, but its nutritional requirements are higher than those of ordinary bacteria. In addition to basic nutrients, 10 ~ 20% human or animal serum should be added to provide cholesterol for mycoplasma. The optimum pH is between 7.8 and 8.0, and if it is lower than 7.0, it will die, but the optimum pH of Ureaplasma urealyticum is between 6.0 and 6.5.
Most of them are facultative anaerobic, and some strains grow well when 5% CO2 is added in the initial separation. Growth is slow, and typical "poached egg-like" colonies appear after 2-3 days incubation on solid medium with less agar content: round (diameter 10 ~ 16um), with thick nucleus and a thin layer of transparent particles around it. In addition, mycoplasma can also grow in chick chorioallantoic membrane or cultured cells.