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What is the purpose and use of cell culture? cell culture
Purpose and use of cell culture:

1, scientific research

Drug research and development, such as new drug screening, vaccines, genetic engineering drugs, cell engineering drugs research and development, monoclonal antibody preparation, etc. Basic research, such as drug action mechanism, gene function, disease mechanism, etc.

2. Biopharmaceuticals

Vaccine production: such as viral vaccine (hepatitis virus vaccine, AIDS vaccine, etc. ) and polypeptide vaccine (tumor vaccine). Production of genetically engineered drugs: such as erythropoietin. Production of antibody drugs and gene therapy drugs.

Cell engineering drug production: some bioactive peptides and bioactive substances in biological cells. The activity of bioactive substances was measured in vitro by cell method. And predict its efficacy in vivo and replace in vivo methods to detect the biological activity of its finished products.

Extended data

The culture of cell culture is a single cell or cell group. The process of cell culture is complex and strict. In order to make cells survive in vitro for a long time, it is necessary to simulate the in vivo environment and supply the materials and energy needed for cell survival, such as water, inorganic salts, amino acids, vitamins, glucose, growth factors and so on. And strictly control culture temperature, environment, pH and other factors, so that cells can survive in vitro.

In addition, there are strict requirements on the cleaning of cell culture products, the preparation and sterilization of culture solution, especially aseptic operation, which is the key to the success or failure of cell culture.

After the first successful passage, the primary cells of this cell line became a cell line, which was composed of the original cell lineage in the primary culture. Cell groups with specific characteristics or markers obtained from primary cultures or cell lines are formed by selection or cloning.

This specificity or symbolism of the cell line must always exist during the whole culture period. Cloning refers to a group of cells with the same genetic characteristics produced by mitosis of the same ancestor cells.