Enterococcus faecalis is a gram-positive and hydrogen peroxide-negative cocci, which is one of the main flora in human and animal intestines. It can produce natural antibiotics, which is beneficial to human health [2]. At the same time, it can also produce bacteriocin and other bacteriostatic substances, inhibit the growth of pathogenic bacteria such as Escherichia coli and Salmonella, and improve the intestinal microenvironment [3]; It can also inhibit the reproduction of urease-producing bacteria and spoilage bacteria in the intestine, reduce the contents of urease and endotoxin in the intestine, and reduce the contents of ammonia and endotoxin in the blood [4]. As a probiotic, Enterococcus faecalis has been widely used in the fields of medicine and food engineering [5-6]. In addition, Enterococcus is a microorganism that normally exists in the digestive tract and has strong tolerance and colonization ability in the intestinal mucosa. It is a facultative anaerobic lactic acid bacteria. Compared with anaerobic Bifidobacterium, it is more suitable for production and application.