There are great differences between animal cells and microbial cells, and there are strict requirements for in vitro culture. For example, animal cells are very sensitive to shear force, so it is impossible to choose a reactor with such high shear force as microbial cells. Therefore, the traditional microbial cell bioreactor needs to be reformed before it is applied to animal bioreactor. Animal cell culture can be divided into three types. One is suspension culture, that is, cells grow in suspension in an incubator, so be brave in non-adherent cells. These cells can grow in vitro without adhering to the wall, and can be suspended in the culture medium by microbial method. The corresponding reactors for this cell culture can be selected as follows: stirred reactor, hollow fiber reactor, ceramic rectangular channel honeycomb reactor and airlift reactor; The other is monolayer culture, which refers to cell culture that must be attached to the surface of solid medium. It is mainly suitable for adherent dependent cells (also called adherent dependent cells), and most animal cells belong to this type. These cells need to be attached to some solid and semi-solid surfaces with appropriate positive charges. After attached growth, cells are no longer in their original form, but are generally single. There are mainly fibroblast type, epithelial type, wandering type and pleomorphism. Bioreactors that can be selected for this kind of cell culture are: stirred reactor (microcarrier culture), glass bead bed reactor, hollow fiber reactor, ceramic rectangular channel honeycomb reactor and so on. The other is immobilized culture, which is suitable for both adherent-dependent cells and non-adherent-dependent cells. It has the advantages of high cell growth density, strong shear resistance and strong pollution resistance. This kind of bioreactor for cell culture includes fluidized bed reactor, solidified bed reactor and so on.