You can also use it in other meristems. 1. In plants, the virus mainly moves through two ways: one is through maintaining the system, and the vascular system has not yet formed in the meristem; Second, through plasmodesmata, the virus moves very slowly, and it is difficult to catch up with the actively growing shoot tips and root tips. 2. In the meristem with vigorous division, the metabolic activity is very high, which makes the virus unable to replicate. 3. The shoot tip contains high levels of endogenous hormones, which can inhibit the proliferation of the virus. 4. There is a virus inactivation system in plants, which has the highest activity in meristem. Therefore, the meristem is not infected. There are four possible reasons. Whichever statement is correct, meristem is usually virus-free. Therefore, using this principle, shoot tip culture was carried out to cultivate virus-free seedlings.

However, just like other organ cultures, in shoot tip culture, explants with no pathogenic bacteria on the surface should be obtained first. Therefore, in general, the shoot apex meristem is closely protected by overlapping leaf primordia, so as long as it is carefully dissected, sterile explants can be obtained without surface disinfection. Compared with root tips, shoot tips are easier to obtain in large quantities and do less harm to plants, so shoot tip culture is often used.