B, before animal cells are cultured, they should be treated with trypsin or collagenase to disperse the cells. B is correct;

C, animal cell culture and animal tissue culture methods are not exactly the same, the former needs trypsin, while the latter does not need trypsin treatment, C is wrong;

D, cell culture technology can't get a complete animal individual, at present, it can only be obtained by nuclear transplantation technology, and D is wrong.

Therefore, choose: B.