The biggest disadvantage of stirred bioreactor for animal cell culture is that it is easy to damage cells due to its large shear force. Although there has been some improvement, this problem is still unavoidable. In contrast, the non-stirred reactor produces less shear force, which shows a strong advantage in animal cell culture.

(1) The filling of packed bed reactor is to fill the reactor with something for cells to grow on the wall. Nutrient solution is provided by circulating perfusion, which can be continuously supplemented during circulation. Oxygen needed for cell growth can also be carried by circulating nutrient solution outside the reactor, so there will be no bubbles to harm cells. This reactor has small shear force and is suitable for high-density cell growth.

(2) Hollow fiber reactor Hollow fiber reactor is widely used in animal cell culture because of its small shear force. This reactor is composed of hollow fiber tubes, each tube has an inner diameter of about 200μm and a wall thickness of 50 ~ 70 μ m, and the tube wall is a porous membrane, through which small molecules such as O2 and CO2 can freely diffuse, and animal cells can grow on the outer wall of the hollow fiber tube, so that oxygen can be easily obtained.

(3) The airliftbioreactor is also one of the commonly used equipment to realize high-density culture of animal cells, which is characterized by simple structure and convenient operation. Some people used microcarrier culture technology in airlift reactor to study the technological conditions of high-density culture of Vero cells. Experiments show that Vero cells can grow normally on the surface of microcarrier when microcarrier is cultured in airlift reactor, and the final density is 1. 13× 106 cells /mL.