(2) Sepals and petals of sterile flower buds should be removed under aseptic conditions. Because the anthers connected with filaments are not conducive to the formation of callus or embryoid, it is necessary to completely remove filaments and try not to damage anthers.
(3) Aseptic technology is the key to plant tissue culture, and high-pressure steam sterilization is generally used to sterilize the culture medium; Generally, 70% alcohol and 0. 1% mercuric chloride solution are used to disinfect explants, but both the disinfection effect of chemicals and the tolerance of plants should be considered. The conditions of tissue culture of Chrysanthemum morifolium are as follows: pH is generally controlled at about 5.8, temperature is controlled at 18 ~ 22℃, and sunlight is 12h. ..
(4) In order to cultivate virus-free orchid plants, the meristem of orchid stem tip should be selected as explants, because there are few or no viruses in this part. In the process of plant tissue culture, auxin and cytokinin can promote cell division and differentiation, and the ratio of them will be different at different stages of culture. In the process of redifferentiation, auxin is high, which promotes root differentiation and cytokinin is high, which promotes bud differentiation.
So the answer is:
(1) Completely unopened magenta acetic acid roasted flower blue chrome alum blue black
(2) Anthers connected with filaments are not conducive to the formation of callus or embryoid.
(3) Tolerance of medicinal plants: autoclaving 18 ~ 22 12.
(4) There are more auxin than cytokinin in shoot apex meristem.