B, most enzymes are protein, which can play a role in vivo or in vitro as long as the conditions are suitable. B is wrong;
C in prokaryotes, there are no real nuclei and nucleoli. But only one organelle, ribosome, C is wrong;
D, when cells are aging, the volume of the nucleus becomes larger, the permeability of the cell membrane changes, and the material transport function is reduced. D is wrong.
So choose: a.