First of all, in the aspect of sturgeon egg collection and insemination. Semen can be collected by squeezing. An individual weighing 20 kg can excrete 30 ml of semen at a time, or even more. Male fish can be used many times. High quality semen is pure milk-like. Take eggs by laparotomy or push them by hand. Individuals with body weight 15kg can lay 2.5 ~ 3.5 kg, about 9 ~120,000 eggs. Semi-dry artificial insemination. The dosage of semen is per kilogram of fish eggs 10mL. When using semen, dilute it with sterile water first. The dilution ratio of semen to water is 1∶200. During insemination, semen is put into fish eggs, stirred evenly for 3 ~ 4 min, so that sperm and eggs are fully combined, left for a period of time, discarded with sewage and washed clean. The eggs of ACIPENSER schrenckii are sticky, which usually appear at 5 ~ 6 min after fertilization and reach the maximum viscosity at 15 ~ 18 min, so the eggs must be debonded before hatching. Most of the debonding agents are talcum powder or fine yellow mud. The former is made into 20% aqueous solution, and the latter is made into 10% concentration.
Finally, the most important thing to pay attention to is the incubation stage. The eggs of ACIPENSER schrenckii are big and heavy, about 40 thousand per kilogram. It is best to hatch under the condition of running water, or stir the eggs regularly. The special incubator is generally 380cm×65cm×30cm, with water inlet and drainage system and regular egg transfer device, which can hatch 400,000 eggs of ACIPENSER schrenckii at one time. When hatching, the water is 50 ~ 60L/min, and the automatic egg transfer device is used once every minute, and the hatching rate is about 85%. In addition, double cages can also be used for hatching. The size of the cage is 80cm×60cm×50cm, and each hatching egg 1kg. The method is to float in the cage in Jiangwan with clear water quality and water flow speed of 0.8 ~ 1.5m/s, and turn the eggs every 20min, and the hatching rate is about 85%. The incubation temperature is 16 ~ 24℃, and the optimum temperature is 19 ~ 22℃.
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