Extract from branches and leaves of mysterious fruit trees, and its extraction method and application. Pulverize that leaves and branch of the mysterious fruit to obtain extraction raw materials; Adding ethanol aqueous solution, reflux extracting for 2-5 hours, filtering, vacuum distilling overnight, freeze drying to obtain dry powder of coarse extract of branches and leaves, dissolving in water, treating with macroporous adsorption resin, eluting with ethanol aqueous solution, respectively concentrating the collected liquids under reduced pressure, and freeze drying to obtain refined extract of branches and leaves; The total phenol content in the obtained extract is 66.5%, and the total phenol content in the branch extract is 84.65438 0%. For DPPH, the scavenging capacity is 7.9 and 7.8 times that of glutathione. The absorption capacity of oxygen free radicals is 7.0 and 6.2 times that of glutathione; Has good xanthine oxidase inhibitory activity; Can be used for preparing antioxidants, anti-gout drugs and health products.