Current location - Health Preservation Learning Network - Slimming men and women - How is it that normal cells always appear trailing in comet electrophoresis?
How is it that normal cells always appear trailing in comet electrophoresis?
In the comet experiment, although there is no special requirement for the carrier of cells-the glass slide, agarose gel is very easy to drift, break and be confused on the ordinary glass slide after spreading the gel on the ordinary glass slide and carrying out multi-step operations such as cell lysis, washing and electrophoresis. In addition, experiments often need to carry out multiple samples and glue on multiple ordinary slides, which is very complicated. The thickness of ordinary glass slides is usually uncontrollable. Due to the different thickness of agarose gel between samples, the morphology of cells is easy to be different when observed under a microscope. When laying low-melting agarose gel containing cells, some low-melting agarose gels often exceed the first layer of ordinary agarose gel and directly contact the glass surface. Because of the effect of glass, cells that come into direct contact with the glass surface will produce false positives. During electrophoresis, the time, voltage and current of cell electrophoresis should be controlled accurately, otherwise the comparability will be affected. When electrophoresis is carried out on multiple samples at the same time, the experimental operation needs to be cautious, the conditions should be strictly controlled, the operation is cumbersome and easy to make mistakes.

In order to solve the inconvenience and error caused by comet experiment slides, special comet slides can be used. The glass slide with specially treated glass surface has the characteristics of close combination with the first layer of ordinary agarose gel; The porous plate material on the glass sheet is very special, hydrophobic and low in static electricity, which avoids the influence on cells and can be well combined with agarose gel. The aperture size and number of orifice plates are selected according to the experimental requirements, which is simple to operate and can be reused.

Specially treated comet experimental slides can reduce errors, improve operability, sample comparability and simplify the process. Let you have a better mood to appreciate the beautiful shapes of some cells in your works, just like a beautiful and mysterious comet galloping in the vast universe, which makes you sigh how amazing life is. Life is actually another universe, and its beauty and true meaning are waiting for you to explore and discover.