The PAR XRS 25 bioreactor system has a unique double-shaft stirring characteristic, that is, it can swing on two horizontal shafts with a 90-degree interval at the same time. Its three-dimensional rotation mode can produce eddy current with low disturbance. Under similar stirring conditions, its mixing efficiency is almost three times that of the traditional stirring system, and its KLa/h value reflects higher mass transfer performance.
For example, in the pH balance experiment of 20L volume,
The traditional stirring system adopts the maximum rotation speed of 42rpm and the maximum angle of 10.4, and it takes 50-98 seconds to stir evenly; Using the 75% Pell XRS 25 bioreactor system with the maximum speed of 30rpm, the maximum long axis angle of 65438 05 and the maximum short axis angle of 65438 0/3 5, the mixing can be completed in only 65438 06 seconds.
According to the determination of nitrogen removal recovery rate, the KLA/H of the traditional stirring system is 42rpm, the maximum speed 10.4, and the swing angle; At the maximum speed and swing angle of 40rpm Parr XRS 25 bioreactor, the major axis is 15, the minor axis is 15, and kla/h is 73.
Better mixing is beneficial to the rapid transfer of nutrients and the removal of metabolic wastes, thus obtaining higher cell concentration and vitality and higher protein expression level. For example, in the culture of CHO (China hamster ovary) cell strain with a volume of 20L, compared with the traditional stirring system, the cell concentration obtained by the Parr XRS 25 bioreactor increased by 18%, and the antibody titer increased by 30%.
Pell XRS 25 bioreactor has been optimized to meet GMP production standards:
1, the biological container made of Allegro film, which integrates optical sensor, optical filter and pre-installed sleeve, is a real disposable system with convenient installation and shorter turnaround time;
2, the whole layer is heated, and the temperature distribution is uniform;
3. The intake and exhaust gas can be filtered and sterilized by 0.2μm, and the integrity test can be carried out;
4, multiple liquid feeding ports, simple feeding operation and easy control of pH value by adding acid/alkali;
5. The bottom discharge is integrated with the rotary lifting valve, which is convenient to use and has less residue;
6. Integrate disposable pH and dissolved oxygen (DO) sensors to reduce the possibility of pollution;
7. Integrated sampling port, sampling can be carried out without stopping stirring, and the residue is less than 5mL.
8. The compact XRS 25 full-function controller adopts SCADA software, which meets the requirements of CFR 21part11.
The double-shaft stirring of the Pell XRS 25 bioreactor system enhances the cell culture performance and provides a more effective choice for 2-25L scale culture.
For more information about the parameters and uses of PAR XRS 25 bioreactor, please contact us at 1352 1452266.
—— Beijing Zeping, an American first-class agent in Beijing.