Haploid breeding: haploid plants are obtained from microspores by anther culture, and normal diploid plants are obtained after chromosome doubling, which is a new breeding method.

2. Embryo culture, ovary culture and ovule culture: In order to overcome the incompatibility of distant hybridization, embryo, ovary, ovule culture and in vitro fertilization can be used.

3. Selection and application of mutants: Because of the success of plant single cell culture, this method can be used to induce single cell mutation. By screening the needed mutants, cells will differentiate into plants, and then they will be stably inherited through sexual generations, which is a way to transform plants at the cellular level.

4. Somatic hybridization and genetic engineering, through the mutual fusion of heterogeneous protoplasts (somatic hybridization), have opened up a new way for plant breeding.

5. Rapid propagation of plants can be achieved through tissue culture.

6. Virus-free plants can be cultivated by tissue culture.

7. The compounds produced by tissue culture include cardiac glycoside, indole alkaloid, berberine, coenzyme Q 10 and other secondary metabolites.

8. Research on artificial seeds.