In April of spring, when the bud swells and the bud scales are not split, the stem tip of 1 1-93 is used as explant and cut into stem segments with growing points. After peeling off phloem, they were soaked in 70% alcohol for 65438 0 minutes and transferred to 0.65438 0% HgCl2 solution.
② subculture
The growing point was inoculated on the culture medium of MS+BA0.5+IAA0.2+GA0.25, and the light intensity was 3000 lux, the daytime temperature was 25-28℃, the nighttime temperature was 18-20℃, the light time was about 10 hour, and the stem tip was10-/kloc. In order to speed up the propagation, the dosage of GA can be increased to 1.0 for repeated cutting culture. After subculture for 6 times, the propagation coefficient of a growing point can reach about 500 times, and it can grow into a cluster seedling with a plant height of 2-3 cm. 1992 grafted 22 shoot tips and cultivated more than 14300 test-tube seedlings. Rootless seedlings, with an average plant height of 2.5 cm and a stem diameter of 0.3 cm.
③ Rooting induction
After the rootless seedlings were subcultured, stems about 2 cm high were cut from 1993 in February and transferred to1/2 ms+IAA1.0; 1/2 ms+IAA 1.5； 1/2 ms+IAA 2.0； 1/2 ms+IAA 1.0+IBA 0.2； 1/2 ms+IBA 0.5+IAA 1.0； 1/2 ms+IBA 0.5； The rooting rates of1/2ms+IAA1.5+NAA 0.2, 1/2MS+IAA2.0+IBA0.3 and 1/2MS+IAA2.0+NAA0.2 were 26.6%-88% respectively. Through comparative experiments, the best rooting medium was1/2 ms+iaa2.0+iba0.3. The rooting rate of test-tube seedlings directly affects the success of tissue culture seedling transplantation. A large number of test-tube seedlings can also grow strong and high-quality roots, 1993 * * * rooting 535 1 plant.
Fig. 8 Tongue joint method
1. Scion 2. Rhizome 3. connect