1. Plant tissue culture is to cultivate in vitro plant organs or tissues into embryoids or plant seedlings under certain conditions, so anther in vitro culture belongs to plant tissue culture.

2. Generally, tissue culture is based on somatic cells, so the traits between offspring and parents are very similar, almost completely following parents, regardless of cytoplasmic inheritance, so it can be regarded as a cloning technology.

In vitro anther culture, the essence of anther culture is to cultivate sperm cells (belonging to germ cells and male gametes), so the nuclear genetic material of new seedlings is half that of their parents, which can be regarded as a special type of sexual reproduction (parthenogenesis). So technically, there is an inclusive relationship between the two.