The virus-free seedlings obtained by shoot tip culture and detection, on the one hand, preserve the original virus-free seedlings; On the one hand, cutting propagation is carried out according to needs, ms culture medium is put in a triangular bottle, and the segments are inoculated into the culture medium for culture. All the above operations are carried out under aseptic conditions. Roots are born in 3-5 days under suitable temperature and light conditions, and grow into small plants in about 20 days, which can be used for cutting propagation or original seed propagation.
Extended data
1, seedling raising
Early-maturing varieties are raised in greenhouse+small arch shed+three layers of thermal insulation plastic film, cultivated with plastic film after early emergence, and supplied to the market in advance; Conventional cultivation can adopt small arch shed+plastic film double-layer insulation seedling raising. The seedbed is about 65438 0.0m wide and 65438 0.5-20cm deep. Cover the bed with organic fertilizer and then water it.
The selection of seed potato requires that it has the typical characteristics of this variety, without pests and diseases, and the potato block100-250g. The planting density is about 3 cm between potato pieces, and the seed potato should be covered with soil, with the thickness of 2-3 cm, not more than 5 cm, so as not to affect the emergence of seedlings.
When 60% of the potato pieces germinate, remove the plastic film. When the temperature is above 20℃ in sunny days, open the two ends of the arch shed membrane and greenhouse membrane for ventilation to prevent high-temperature seedling burning, and keep the bed temperature at 25-30℃, and the humidity shall be subject to the humidity of the bed soil. When potato seedlings grow to 20-25 cm and have 6-8 intact leaves, they can be cut and planted in the field.
2. Planting
When the average temperature is above 65438 05℃, the seedlings can be cut and planted in the field. Because of the low temperature in the early stage (from late April to mid-May), plastic film mulching can be used for cultivation. When planting, four nodes are horizontally or obliquely inserted into the soil. In drought, two leaves are exposed to the ground with one heart and the rest are buried in the soil, which is beneficial to the survival of potato seedlings and the uniform dispersion of potatoes, and improves the commodity rate and yield. The planting density is 4000 plants /667 square meters, and the spacing between plants is 20 cm.
Baidu encyclopedia-sweet potato
Baidu Encyclopedia-Virus-free Sweet Potato