Seedling raising method of vegetative cells
A method for culturing seedlings by enzymolysis of laver vegetative cells. Homogenize algae-eating conch, adjust PH, and add glucose or sucrose to prepare laver vegetative cell dissociation solution. The thallus of Porphyra yezoensis was placed in dissociation solution to prepare vegetative cells, which were cultured in seawater for 10- 15 days for artificial seedling collection, and the sexual reproduction technology of Porphyra yezoensis was changed to vegetative cell seedling raising. The invention has the advantages that: the period of raising laver seedlings is shortened from 6 months to about 15 days; Secondly, it solves the difficulty of raising seedlings of Porphyra haitanensis in the north, so that Porphyra haitanensis can be cultivated in the north; The third method is a means of cultivating laver. The invention belongs to the economic seaweed seedling raising technology, and is a method for artificially cultivating laver by using enzymatic method to dissociate laver thallus to obtain single vegetative cell. The invention is characterized in that the soft part of algae-eating conch is homogenized, the PH value is adjusted, protein and harmful enzymes are removed to prepare an enzyme solution, and 1.5-2 mol sugar is added to the enzyme solution to prepare a laver vegetative cell dissociation solution. Vegetative cells can be decomposed by enzymes. The dissociated single cells were washed, cultured for 10- 15 days, picked with a seedling picker for 1-2 days, and then cultured in the sea. This method is simple and easy to popularize, and it is an asexual propagation and seedling raising method.