"-generation" refers to a period of time from cell inoculation to the next subculture inoculation.

Delay (slow)

Free phase: the cells are suspended in the culture solution after inoculation, and the cell body is spherical. General 10 minutes to 4 hours.

Adhesion period: adhesion and gradual extension-generally within 24 hours.

Latency period; At this time, the cells have growth activities, but there is no cell division. The incubation period of cell lines is usually 6-24 hours.

Exponential growth period (rapid proliferation)

The number of cells changes with time, the star index increases, and the vitality is the best, which is most suitable for experimental research.

Stagnation (stop of growth):

When the number of cells reaches the saturation density, the cells stop proliferating.