The value of SOD is high. Although SOD is an enzyme, it plays an antioxidant role in organisms. Superoxide free radical is a highly active oxidizing substance produced in the process of oxygen metabolism, which can damage cell membrane, DNA, protein and other important biomolecules and participate in the occurrence and development of many diseases. The main function of SOD is to degrade superoxide radicals, thus maintaining the redox balance in cells and protecting cells from oxidative stress.

SOD plays an important role in many biological processes, including antioxidant defense, anti-inflammatory response and apoptosis regulation. It exists widely in many organisms, including animals, plants and microorganisms. Sods in different organisms have different structures and characteristics, which are mainly divided into three forms: cytoplasmic Cu/ZnSOD, mitochondrial MnSOD and extracellular EcSOD.

Matters needing attention in preparing SOD

1. Laboratory safety: Before any laboratory work, make sure to observe the laboratory safety regulations and operating procedures. Wear appropriate personal protective equipment, such as laboratory gloves, laboratory coats and goggles.

2. Material preparation: prepare the required materials, including culture medium, cultured cells or microorganisms, Petri dishes, culture equipment and reagents. Ensure that the materials and reagents used are of high purity and sterile, and are stored and treated according to the instructions.

3. Cell culture: If cell culture is needed to prepare SOD, the cell culture environment should be clean and sterile to ensure the health and purity of cells. Follow the standard operating procedures of cell culture, such as changing the culture medium regularly and detecting the growth state of cells.

4. protein extraction: According to the experimental scheme, SOD protein was extracted by appropriate methods. This may involve cell lysis, centrifugation, column chromatography or other techniques. Ensure that the degradation and pollution of protein are avoided in the extraction process.

5. Purification and concentration: Purify and concentrate the extracted protein to obtain high-purity and high-concentration SOD. Use appropriate purification techniques, such as ion exchange chromatography, gel filtration chromatography, affinity chromatography, etc.

Reference to the above content: Baidu Encyclopedia -SDO