Steps: (1) Inoculate the anthers whose pollen has developed to a certain stage on artificial culture medium, and carry out in vitro culture by aseptic operation technology; (2) Pollen can divide many times under the specific conditions provided by the culture medium to form embryoid structure (embryoid) or callus; (3) Inducing callus to differentiate into buds and roots, and finally growing into plants. Materials: Generally, it is the anther of mononuclear pollen (microspore) cultured in vitro. Through culture, it can differentiate into haploid plants without the normal development path (that is, the way to form mature pollen and eventually produce sperm). Conclusion: Totipotency of plant cells: Care must be taken not to damage anthers during the whole operation. If it is damaged, it should be eliminated, because the damage often stimulates the pollen wall to form diploid callus.