2, for the diagnosis and treatment of various diseases (such as wound repair and healing, cancer diagnosis and treatment, etc. ).
As a biochemical reagent
It provides a foundation for the industrial application of cell culture and bioengineering. Among the many functions of FN, the most basic and important one is to promote cell adhesion and growth, and cell adhesion is a necessary condition to maintain the body structure and complete cell growth. Therefore, FN is one of the important components of cell culture medium. Zhengzhou Dewen Biotechnology Co., Ltd. and China Academy of Medical Sciences jointly applied porcine plasma FN. The results show that FN, as the substrate of cell culture, can make the adherent rate of various cells reach more than 90% (compared with 10% in the control group), the cell morphology and structure are good, the cell metabolic rate is enhanced, the synthesis speed of DNA, RNA and protein is increased by about 5 times, the cell colony rate is increased by several times, and the survival rate of primary culture is obviously improved. Studies have also proved that FN can improve the cell fusion rate and shorten the fusion cycle, and can be applied to many scientific research, such as cell hybridoma technology. Wrapping FN on microspheres as a culture medium for mass production of cells can save space and raw materials, and become the basic substance for producing new drugs by cell culture technology. Cell culture technology is one of the methods often used in biological research and one of the indispensable means of bioengineering. The development of FN products will play a positive role in promoting the wide application of cell culture and the application and development of bioengineering. The application of FN as biochemical reagent will increase day by day, and the market potential is huge. At present, FN biochemical reagents are mainly produced by Sigma Company (human, bovine and mouse plasma FN, etc.). ), and their application in China mainly depends on imports. Therefore, it is urgent to develop FN biochemical reagents in China (Zhengzhou Dewen Biotechnology Co., Ltd. has developed FN biochemical reagents and obtained relevant national patents).
Used in the diagnosis and treatment of various diseases, wound repair and healing: A large number of studies and clinical applications have proved that FN plays a very important role in wound repair and healing and is the key substance to promote wound healing. At present, the application of human plasma FN in burn, trauma, septicemia and infection has been studied abroad. Nishida proved by animal experiments that the healing speed of FN coated on rat skin wound was obviously accelerated. Patients with nutritional corneal ulcer were treated with some media mixed with FN in the form of eye drops. After 2 days, the epidermis began to proliferate. After three weeks, the ulcer disappeared completely, with no scar and no side effects. When FN is applied to the ulcer, it can combine with collagen in the wound to form a protective layer of FN, which is not only beneficial to the migration and proliferation of epithelial cells to repair the wound, but also can prevent the infection of germs. Scovil experimental group found that the supplement of FN to some trauma patients played a positive role in improving peripheral blood vessels and lung function, and the clearance rate of foreign bodies in patients' wounds increased and the infection rate decreased. The research on the application of FN in China started late, and the research results of the Institute of Basic Medicine of China Academy of Medical Sciences and other units also proved that the wound healing time of FN as an external ointment for treating mechanical wounds and burns in rats was shorter than that of the control group 1/3- 1/2, and there was no infection, scar and any side effects. Recently, the Burn Department of the Fifth Hospital of Harbin applied plasma extract rich in FN to 46 burn patients. Their clinical trials have also proved that applying FN to the burn site can greatly shorten the course of treatment and reduce scar healing, especially for deep burn patients. The experimental results of rats in Kunming General Hospital of Chengdu Military Region showed that the average length of regenerated epidermis in the experimental group was 89.27um, which was significantly different from that in the control group. The experiment of "external application of FN to promote burn wound healing in mice" was completed by the Burn Institute of Shanghai Changhai Hospital. The results showed that the average healing time of wounds in FN group was 65438 08.67 days, while that in control group was 22.89 days, with significant difference between the two groups. In addition, the Ophthalmology Center of Sun Yat-sen Medical University, Shanghai Second Medical University, Guangzhou Red Cross Hospital and other units have applied FN to treat refractory corneal epithelial injury, keratitis and other ophthalmic diseases and achieved remarkable results. 2. Diagnosis and treatment of cancer: A large number of studies show that the distribution and content of FN in the body are closely related to the occurrence and development of tumors. At present, the relationship between FN and tumor has become more and more well known, and the research on FN is also deepening. It will be a very valuable project to develop FN products for the diagnosis and treatment of tumors.
3. Treating diseases of blood system and cardiovascular and cerebrovascular system: Experiments have proved that supplementing plasma FN to the blood of patients with EDS can achieve platelet aggregation and alleviate diseases. Atherosclerosis is a disease that generally harms the health of middle-aged and elderly people. It has been proved that FN participated in this process. With the aggravation of vascular sclerosis, the content of FN decreased. The deficiency of plasma FN will lead to the destruction of microvascular integrity, increased permeability, massive extravasation of plasma, blood concentration, increased viscosity, decreased effective circulating blood volume, imbalance between plasma and tissue fluid, and body shock.
4. Adjuvant treatment of first aid such as sepsis and shock: When a large area of trauma and infection occurs in the body accompanied by sepsis and shock, the level of FN in the patient's blood drops sharply, making the body extremely weak and unable to resist external infection. After timely supplementation of exogenous FN, the patient's condition will be relieved. There are many clinical studies abroad. Robbins and others, for example, have no effect on trauma complicated with sepsis and lung infection. When FN was used, the lung function was improved, the patient's resistance was enhanced and the wound healing was accelerated. Toddy and others selected 93 patients with severe septicemia from Icu and gave them FN, which improved the cure rate and prolonged the survival time of critically ill patients. In 1990s, clinical research in China was active. For example, Tianjin First Central Hospital and Hunan Medical University pointed out that the decrease of plasma FN level was one of the main reasons for sepsis, shock, burn and trauma with multiple organ failure. After FN supplementation, it can increase the clearance of particulate matter in blood by reticuloendothelial system and improve microcirculation function. Improve the glomerular filtration rate of patients with renal insufficiency; It can reduce the blood lactic acid concentration of critically ill patients and increase the arterial oxygen partial pressure.
5. Relationship between FN and diabetes and other diseases: According to domestic and foreign research reports, the synthesis of extracellular matrix protein in diabetic patients has changed, and the ratio of FN to collagen has decreased, that is, the relative content of collagen has increased. Herrick et al. pointed out that the content of FN decreased in diabetes and venous ulcer of lower limbs. The experimental results of the 304th Hospital of China People's Liberation Army also confirmed this view, that is, the decrease of FN content in diabetic ulcer wounds is one of the reasons for the long-term nonunion of wounds. In addition, many studies have proved that FN is related to liver diseases. FN exists in the basement membrane of hepatic blood vessels, lymphatic vessels and bile ducts, around nerve axons and in the hepatic sinuses of normal liver tissues. The content of FN in plasma of patients with fatty liver, hepatitis and cirrhosis is twice that of normal people. It is also found that the content of FN in synovial fluid of patients with rheumatic joints is higher than the normal level, indicating that FN is closely related to the occurrence and development of various diseases. The sampling test results of plasma FN content in healthy elderly and adults over 60 years old in the Affiliated Hospital of Shanghai Second Medical College show that the plasma FN value in the elderly group is significantly lower than that in the adult group, which may be one of the reasons why the elderly are prone to infection, tumor and thromboembolic diseases.