The low ability of Taxus cell line to synthesize taxol and unstable passage are the main reasons that limit its large-scale culture. So far, taxol production by Taxus cell culture has reached the pilot level, but to achieve large-scale production, the key is to improve the culture yield and taxol content and select high-yield cell lines. Therefore, the establishment of a stable high-yield cell line and its stable preservation technology is the premise of industrial production.

The poor ability of Taxus clones to integrate taxol and the unstable passage are the main reasons that limit their large-scale cultivation. Up to now, taxol production by Taxus clones has reached the pilot level, but the key to realize large-scale production is to improve the yield of culture and taxol by selecting high-yield clones. Therefore, the premise of industrial production is to establish stable high-yield cell lines and stable storage technology.

The purpose of this study is to screen taxol-producing cell lines by adding precursors and ultraviolet mutagenesis. The main results are as follows:

The purpose of this study is to screen cell lines with high taxol production from ultraviolet mutation by increasing precursor screening. The main results obtained are as follows:

(1) Taxus cells have high tolerance to the precursor phenylalanine, and the effect is slow and lasts for a long time. When the concentration of phenylalanine reached 4.0mmol/L, the subculture stopped growing. Therefore, the optimal screening concentration is 3.0 mmol/L.

(1) Taxus cells have a relatively high level of tolerance to the precursor phenylalanine, and the effect is quite slow and lasts for a long time. When the concentration of phenylalanine reaches 4.0 mmol/l, subculture can't grow, so the optimum concentration for filtration is 3.0 mmol/L.

(2) The tolerance of Taxus cells to the precursor sodium benzoate is low, and the effect is rapid and short-lasting. When the concentration of sodium benzoate was 0.5mmol/L, the cells grew well, but the promotion effect was not obvious. When the concentration of sodium benzoate was 1.5mmol/L, the cells could not grow. When the concentration of sodium benzoate is 1.0mmol/L, subculture can not grow again, and when the concentration of sodium benzoate is 0.5mmol/L, subculture can grow again, so the optimal screening concentration is about 0.5 mmol/L.

(2) The tolerance of Taxus cells to precursor benzoate is relatively low, and it is affected quickly and for a short time. When the concentration of benzoate was 0.5mmol/L, the cells grew well, but the promotion effect was not obvious. When the concentration of benzoate reached 1.5mmol/ L, the cells stopped growing. When the concentration of benzoate reached 1.5mmol/L, subculture could not grow, but grew at 0.5 mmol/L. Therefore, the ideal concentration of filtration was about 0.5 mmol/L.

(3) It is feasible to screen high-yield cell lines of Taxus by UV mutagenesis combined with precursors. When sodium benzoate was 0 mmol/L, the cells of Taxus began to grow. But it is random to some extent, so it is uncertain.

It is possible to obtain high-yield Taxus cell lines by combining ultraviolet mutagenesis with precursor screening, because new Taxus cells grow when the concentration of benzoic acid is 0mmol/L; However, due to randomness, it has not been confirmed.