Primary cultured cells will divide. Generally, primary cultured cells will not be easily transmitted to about 65,438+00 generations, and the growth of cells will stagnate, and most cells will age and die. Generally, subculture will pass to 40-50 generations, which is called cell strain. The genetic material of the cell line has not changed. Its mitotic ability is the same as that of primary culture. When the cell line is passed to the 50 th generation, there will be a "crisis" and it can't be passed on any more.