Trihydroxymethyl aminomethane (Tris) is a widely used organic reagent, and its effective buffering range is between pH 7.0 and 9.2. It and its derivatives are widely used in the preparation of buffers in biochemical and molecular biology experiments, and can be used to make drugs, organic synthesis, biological buffers and so on.

Among them, in biochemical experiments, many experiments related to protein and nucleic acid need to use Tris as biological buffer. There is even a tendency to exceed phosphate buffer, because it has good buffering capacity, low toxicity, extremely low interference to biological reactions and high purity. There is a tendency to exceed phosphate. For example, TRIS buffer has been tested in SDS- polyacrylamide gel electrophoresis, and phosphate is rarely used.

Application of tris (trimethylaminomethane)

① The buffer system composed of glycine and electrophoresis buffer is used to stabilize the P value;

(2) using TRIS-HCL buffer system to stabilize the PH value in the gel;

③ Widely used as a solvent for nucleic acid and protein;

The low ionic strength of TRIS buffer can also be used to form intermediate fibers of nematodes;

⑤ Adding EDTA into TRIS hydrochloric acid buffer to make "TE buffer", which can be used to stabilize and preserve DNA;

⑥ Use acetic acid instead of acidic solution to get "TAE buffer", and use boric acid instead to get TBE buffer. These two solutions are commonly used in nucleic acid electrophoresis.