1, stirring, in the culture of photosynthetic bacteria, keeping the bacterial liquid in a dynamic state is beneficial to the floating of bacterial cells to obtain light and promote the good growth of bacterial cells. Practice has proved that the concentration of bacterial liquid cultured in dynamic environment is higher than that in static environment. Usually, the culture solution is slowly circulated by manual periodic stirring or sealed circulating pump.

2, adjust the light, photosynthetic bacteria mostly use infrared light close to 700~900 nanometers, and generally use 25~60 watts of ordinary light bulbs to meet. Different light intensities have different requirements for bacteria. Generally, it is adjusted by controlling the distance between the light bulb and the bacterial liquid level, which is generally controlled at 15~25 cm. Of course, sunshine is the best and there is no cost. Adjusting temperature Generally speaking, the optimum range of strain culture temperature at present is 25~34*C (the adaptive range is 15~40*C). When the temperature is lower than 15%C, the growth is slow, the color of bacterial liquid becomes dark and the precipitation increases.

3. Determination of pH value, because in the process of culture, bacteria use organic acids in the culture solution, which leads to the increase of pH value ... So, at least 1~2 times a day should be measured, initially 1 time a day, and then 1 time can be increased in the next few days.

4. Control of enemy pests. Prevention is the main method to control enemy harm. The main preventive measures are: selecting clean water and strictly disinfecting. Select high-quality strains. The activity of aging photosynthetic bacteria is poor, and there are many harmful metabolites in the bacterial liquid.

5, strains should choose high concentration, strong activity, less mixed bacteria, bright purple bacteria solution. The inoculation amount is dominant and the quantitative advantage is maintained. In the process of large-scale production, because the mixed bacteria in water source, container and air are inevitable, the inoculation amount should be increased to make photosynthetic bacteria take the absolute advantage, so as to cultivate high-quality bacterial liquid.

6. The content of photosynthetic bacteria is high, and it can also release bacteriostatic enzymes to inhibit the growth of some miscellaneous bacteria. Practice has proved that the normal inoculation amount of productive culture should be no less than 1: 5, that is, 1 strain (3 billion grade) and 5 culture solutions; When the inoculation conditions are not good, the inoculation amount should be no less than 1: 1. Choosing suitable culture medium is also an important measure to maintain quantitative advantage.