Bioactive peptides are peptide compounds that are beneficial to the life activities of organisms or have physiological functions. They are a kind of polypeptides with a relative molecular weight of less than 6000Da, which have many biological functions.

Bioactive peptides have a variety of human metabolism and physiological regulation functions, are easy to digest and absorb, and have the functions of promoting immunity, regulating hormones, resisting bacteria and viruses, lowering blood pressure and reducing blood fat. They are the hottest research topics and functional factors with great development prospects in the international food industry.

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There are three main methods to synthesize bioactive peptides artificially:

Chemical synthesis, enzyme synthesis and recombinant DNA? Technology. The main ways of chemical synthesis are solid-phase synthesis and liquid-phase synthesis. The disadvantages of chemical synthesis are racemization and side reactions; The groups on the side chain of peptide must be protected; Excessive connecting reagents and acyl carriers are needed; The toxic residue of the connecting reagent will affect the product quality and the environment.

Enzymatic synthesis has the advantages of mild reaction and oriented catalytic position of enzyme, but there are many by-products and low yield. However, only macromolecular peptides and protein can be synthesized by DNA recombination technology, which is not suitable for small peptides with nutritional value which are mainly needed by human beings.

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