What you want to ask is that the main difference between autotrophic and heterotrophic is whether carbon dioxide can be used, right? That's right.

Autotrophic organisms can use inorganic carbon, such as carbon dioxide, as a carbon source for synthesis, and convert inorganic substances into organic substances through photosynthesis or chemical synthesis. However, heterotrophs cannot use carbon dioxide as a carbon source, but can only use organic matter as energy and synthetic raw materials. Therefore, the main difference between autotrophic and heterotrophic is whether carbon dioxide can be used.