1) is convenient to use, saves cell washing, and does not need radioisotopes and organic solvents;

2)CCK-8 method can be used for rapid detection;

3) The detection sensitivity of CCK-8 method is very high, and even lower cell density can be determined;

4) The repeatability of CCK-8 method is better than MTT method;

5)CCK-8 method is less toxic to cells;

6)CCK-8 cell activity detection reagent contains 1 bottle solution, which can be used immediately without prefabrication.

Of course, compared with MTT, CCK8 has the following two disadvantages:

1) Compared with MTT method, CCK8 and XTT are more expensive.

2)2)CCK 8 reagent is reddish, which is close to the color of the medium containing phenol red. If you don't pay attention, it is easy to miss or add more.

In order to better understand the detection methods such as CCK8, MTT, XTT, etc., the experts of Zisha biological cell biology products made the following table for reference: